Title: 
            Remediation of Concrete Using Microorganisms
        
        
            Author(s): 
             Santhosh K. Ramachandran, V. Ramakrishnan, and Sookie S. Bang
        
        
            
                Publication: 
                Materials Journal
            
            
                Volume: 
                98
            
            
                Issue: 
                1
            
            
                Appears on pages(s): 
                3-9
            
            
                Keywords: 
                calcite; compressive strength; cracking; stiffness
            
            
                DOI: 
                10.14359/10154
            
        
        
            Date: 
            1/1/2001
        
        
            Abstract:
            This paper describes an innovative biotechnology utilizing microbiologically-induced mineral precipitation for concrete remediation. Calcite precipitation induced by Bacillus pasteurii was studied in two types of portland cement mortar specimens: one prepared from mixing with microorganisms, and the other with simulated cracks filled with microbial mixtures. The study showed that there was a significant increase in compressive strength of the portland cement mortar cubes containing lower concentrations of live cells. Compressive strengths of the cubes containing live or dead cell mass, however, decreased as cell concentrations and curing time increased, suggesting the interference of mortar integrity by biomass. Cracks filled with bacteria and sand demonstrated a significant increase in compressive strength and stiffness values when compared with those without cells. Scanning electron micrographs identified that microbiological calcite precipitation occurred mainly close to the surface areas of the crack, where a dense growth of calcite crystals embedded with cells was observed.